Increased ATP and creatine phosphate turnover in phagocytosing mouse peritoneal macrophages.

Differential turnover of phospholipid acyl groups in mouse peritoneal macrophages.

Phospholipid acyl turnover was assessed in mouse peritoneal exudate cells which consisted primarily of macrophages. The cells were incubated for up to 5 h in media containing 40% H218O, and uptake of 18O into ester carbonyls of phospholipids was determined by gas chromatography-mass spectrometry of hydrogenated methyl esters. The uptake was highest in choline phospholipids and phosphatidylinosi...

748 Creatine Kinase and Creatine Phosphate in Macrophages

Creatine phosphate serves as a reuseable high energy phosphate reservoir in skeletal and cardiac muscle and in the brain. These stores of creatine phosphate are thought to rephosphorylate the ATP hydrolyzed during cellular work (see 1 and 2 for review). Studies in our laboratory (3) have shown that mononuclear phagocytes obtained from the peritoneal cavities of mice also contain large stores of...

Mycobacterium leprae in Mouse Peritoneal Macrophages

Mycobacterium leprae synthesizes large quantities of a specific phthiocerol-containing phenolic glycolipid in vivo. We had shown earlier that viable M. leprae readily incorporates radiolabeled palmitic acid into phenolic glycolipid I when residing in cultured macrophages in vitro and that this process is inhibited by the antileprosy drug rifampin. In the present paper we report the application ...

Transport of phagosomes in mouse peritoneal macrophages.

Mouse macrophages were elicited by the peritoneal injection of chondroitin sulfate solution, harvested and purified, and used as experimental materials. Small and large (diameter: 0.9 microns and 3.0 microns, respectively) polystyrene beads (PB) were used as ingested particles. When the macrophages were incubated with Hank's solution containing small or large PB for 30 min, the phagosomes conta...

Creatine kinase: ATP contaminating creatine phosphate substrate of a commercial kit.